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„Influence of seminal plasma on the motility of cooled stallion semen after a highly concentrated storage“ The aim of the present study has been the development of a semen processing method allowing the storage of equine semen at a high concentration of 333 million sperm/ml and low volume. Different seminal plasma concentrations were tested to achieve optimum spermatozoal motility. The quality of the new semen processing method (protocol 3) was evaluated by comparison with two standard conservation methods often used in the routine laboratory (protocol 1 + 2). Protocol 1 is one of the most commonly used semen processing methods due to its simplicity in implementation. The native semen was diluted directly after collection to 25 million sperm/ml. Protocol 2 is also a frequently applied standard semen conservation method. After dilution 1 + 1 with a semen extender and centrifugation at a force of 600 g for 10 min the bulk of the seminal plasma was removed. The sperm pellet was resuspended and diluted to a concentration of 25 million sperm/ml. Protocol 3 represents the new semen preservation method. The first steps were identical with protocol 2 and included a dilution 1 + 1, semen centrifugation at 600 g for 10 min and the removal of seminal plasma. Unlike protocol 2 the sperm pellet was then diluted to 333 million sperm/ml. Semen storage at that high concentration saves a volume up to 92.5 % compared with the two conventional semen processing methods. After storage the highly concentrated semen was diluted to a final concentration of 25 million sperm/ml. Various concentrations of seminal plasma were added to the extender to investigate the influence of seminal plasma on spermatozoal motility. The study was conducted with 8 stallions of different breeds aged from 3 - 12 years. Six ejaculates of each stallion were used for the experiment. All semen preservation methods were performed with four different extenders. Semen storage took place at the standard temperature for cooled stallion semen at 5 °C. Sperm motility was evaluated subjectively after 0, 24 and 48 h of storage. The present study demonstrates that highly concentrated semen can be used for artificial insemination. The new method is a suitable volume-saving alternative to the conventional semen preservation protocols. A small proportion of autologous seminal plasma in the insemination dose may be beneficial in regard to the multiple functions of seminal plasma in the female reproductive tract. Concerning the spermatozoal movement, there is in the majority of cases no difference between portions without seminal plasma and portions with a small amount of 5 or 20 %. Only a high concentration of 80 % seminal plasma should be avoided because of its negative influence on sperm motility. From the extenders tested in this study, INRA 96 yielded the best results with regard to sperm motility. BotuSemen and Gent are also well suited for the shipment of equine semen, however they were most times inferior to INRA 96. EquiPro is the extender that preserves spermatozoal movement worst over a period of 48 h. The study revealed great differences in semen quality among the individual stallions. Therefore it is recommendable to adapt the conservation method, the seminal plasma concentration and the extender type to the individual sire.
Acquisto del libro
Einfluss von Seminalplasma auf die Motilität von gekühltem Hengstsamen nach einer hochkonzentrierten Lagerung, Julia Anna Kristin Lübke
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- Pubblicato
- 2016
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