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Focusing on the development of single-particle cryo-electron microscopy (cryo-EM), the book showcases 55 pivotal articles detailing advancements in methods and significant findings related to biological macromolecules. It emphasizes the technique's versatility, requiring no specific symmetries or arrangements, and highlights applications primarily in ribosome structure, membrane channels, and their mechanisms. A commentary sets the stage by discussing conceptual evolution and collaborative contributions, while the latter sections introduce near-atomic resolution structures and innovative computational analysis techniques.
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SINGLE-PARTICLE CRYO-ELECTRON MICROSCOPY, Joachim Frank
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- 2018
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