D. Lansing Taylor Libri

Only recently has the true power of fluorescence techniques evolved for use with single living cells. The present status of the field reflects the occurrence of a revolution in cell biological research. Volume 30, along with Volume 29, provide the cell biologist with a sourcebook of methods. Volume 29 deals with the preparation, delivery, and detection of fluorescent probes. Volume 30 explores a combination of the theoretical and technical issues related to the quantitation of fluorescence signals in the living cell with a light microscope.

Parts A and B, companion volumes on fluorescence microscopy, are sourcebooks of methods for living cells. Part A comprehensively covers fluorescent analogs, procedures for fluorescent labeling of various cellular structures, and fluorescence microscopy. Part B describes instrumentation and quantitation analysis for imaging and spectroscopy.Key Features* Convenient bench-top formatComprehensive methods inluded * Tracing proteins, peptides, hormones, and toxins* Labeling cell surfaces, mitochondria, endoplasmic reticulum, and endocytic compartments* Preparation of fluorescent probes and their incorporation into cells* Culturing cells under the microscope* Basic fluorescence microscopy and imaging techniques