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Focusing on atomic force microscopy (AFM), this book explores its applications in studying biomolecules and cells, highlighting its unique ability to analyze living systems in aqueous environments with high resolution. It delves into AFM methodologies for biomedical detection, covers a variety of biological systems, and discusses the integration of AFM with complementary techniques. The state-of-the-art chapters aim to equip researchers with the tools necessary to tackle complex biological challenges using AFM.